單核細胞趨化蛋白5抗體說明書

產(chǎn)品名稱：單核細胞趨化蛋白5抗體

英文名稱：Anti-CCL12/MCP5

別名：C-C motif chemokine 12; CCL12; MCP 5; MCP-5; Macrophage/monocyte Chemotactic Protein; OTT

交叉反應：hu, mo, rat, Rb, dog, hrs, cow

產(chǎn)品類型：一抗

應用領域:/腫瘤/免疫學/轉錄調節(jié)因子

蛋白分子：predicted molecular weight: 11kDa

濃度值：1mg/1ml

物理性狀：Lyophilized or Liquid

免疫原：KLH conjugated synthetic peptide derived from human MCP-5 C-terminus

亞型：polyclonal

純化方法：affinity purified by Protein A

保存液：0.01M PBS, pH 7.4 with 10 mg/ml BSA ａnd 0.1% Sodium azide

應用技術：//WB=1:100-500//ELISA=1:500-1000//IP=1:20-100//IHC-P=1:100-500//IHC-F=1:100-500//IF=1:100-500（石蠟切片需做抗原修復）/not yet tested in other applications./optimal dilutions/concentrations should be determined by the end user.

保存環(huán)境：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month ａnd for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

產(chǎn)品介紹：Chemotactic factor that attracts monocytes, lymphocytes, basophils ａnd eosinophils, but not neutrophils. Signals through CCR2B ａnd CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic ａnd non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens.

抗體,也叫免疫球蛋白 (Ig),是一種能特異性結合抗原的糖蛋白,而抗原是在易感染動物體內(nèi)引發(fā)抗體產(chǎn)生的物質。在體內(nèi),抗體是由于外 源性分子的侵襲而產(chǎn)生的?？贵w以一個或者多個 Y 字形單體存在,每個 Y 字形單體由 4 條多肽鏈組成,包含兩條相同的重鏈和兩條相同的輕 鏈。輕鏈和重鏈是根據(jù)它們的分子量大小來命名的。Y 字形結構的頂端是可變區(qū),為抗原結合部位。

任何一個抗體的輕鏈都可以分為昊螂型(基于小分子多肽結構上的差異),每一個抗體的重鏈則決定了它的類或型?？贵w制備是指通向抗體生成的步驟，包括抗原樣品的制備以及將其安全注入實驗動物或家畜體內(nèi)，并由此誘發(fā)抗原特異性抗體在血清內(nèi)達到高表達水平，然后可從該動物體內(nèi)回收抗體。